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. 2016 Aug 30;7:1246. doi: 10.3389/fpls.2016.01246

FIGURE 8.

FIGURE 8

Feeding of P. scaber on etiolated WT (white columns), AtKunitz-PI;1 mutant (black bars) and 35S::Kunitz-PI;1 overexpressor (gray columns) seedlings. In parallel assays, feeding was tested for seedlings that had been grown on agar plates containing the ethylene precursor ACC, ethylene biosynthesis inhibitor silver nitrate, or the auxin IAA. Mock plates (control, Ctr) lacked any additives. Isopod feeding activity was scored by counting the number of damaged versus undamaged seedlings and is expressed as number of undamaged seedlings in the population of 120 seedlings analyzed. Error bars refer to three independent experiments. Asterisks indicate statistically significant changes compared with the corresponding WT sample for each condition calculated by a two-tailed Student’s t-test; ∗∗P < 0.01, ∗∗∗P < 0.001 (cf. Supplementary Table S1).