Table 1.
CaSR expression and putative functions in healthy reproductive tissues.
| Organ/Tissue | Species | mRNA | Anti-CaSR antibody | Protein WB | Protein IFM/IHC | Function related to CaSR | References |
|---|---|---|---|---|---|---|---|
| MALE REPRODUCTIVE SYSTEM | |||||||
| Rat | Testis Epididymis | Rabbit anti-CaSR Acris (N-terminal domain) | Spermatogonia Spermatocytes Spermatids Sertoli cells Epididymal cells | Sperm motility ↑ | Mendoza et al., 2012 | ||
| Horse | Goat anti CaSR (F19) Santa Cruz (N-terminal domain) | Sperm 100 kDa + 77 kDa | Sperms | Sperm capacitation ↓ Sperm motility ↑ | Macías-García et al., 2016 | ||
| Human | Rabbit anti-CaSR Sigma (N-terminal domain) | Epithelial cells in normal prostate tissue | Feng et al., 2014 | ||||
| OVAR | |||||||
| Human | Ovarian surface epithelial cell lines | Polyclonal anti-CaSR Affinity BioReagents | Ovarian surface epithelial cell lines,120 kDa | Pro-liferation ↑ ↑ IP3 ↑ | McNeil et al., 1998 | ||
| Human | Rabit anti-CaSR (C0117-15) US Biological (C-terminal) | Denuded oocyte (metaphase II),130 kDa Granulosa cells of cumuli oophori cells, 120 + 130 kDa | Oocyte (GV, MI, MII state) | Dell'Aquila et al., 2006 | |||
| Horse | Rabit anti-CaSR (C0117-15) US Biological (C-terminal) | Denuded oocyte (metaphase II), 130 kDa Granulosa cells of cumuli oophori cells, 120 + 130 kDa | Oocyte (metaphase II) Granulosa cells of cumuli oophori cells | Oocyte maturation↑ | De Santis et al., 2009 | ||
| Pig | Ocytes Granulosa cells of Cumuli oophori | Goat anti-CaSR Santa Cruz | Oocyte, 160 kDa Somatic cell, 160 kDa | Oocyte maturation↑ | Liu et al., 2015 | ||
| Japanese Quail | Mouse anti-CaSR Abcam or NPS Pharmaceutical (Extracellular domain) | Granulosa explants 115–125 kDa +100–110 kDa | Granulosa cells of preovulatory follicles Granulosa layer after ovulation | Survival↑ (Decreased apoptosis) | Diez-Fraile et al., 2010 | ||
| UTERUS | |||||||
| Rat | Uterine luminal epithelium Uterine stromal cells | Rabbit anti-CaSR Affinity BioReagents | Implanting blastocyst Luminal and glandular epithelium Subluminal uterine stromal cells at implantation site | Blastocyst implantation Decidua-lization | Xiao et al., 2005 | ||
| Rat | Anti-CaSR Affinity BioReagents | Uterine luminal Epithelium Longitudinal and circular smooth muscle layers Blood vessels Luminal and glandular epithelium of the endometrium | Relaxation? | Pistilli et al., 2012 | |||
| Human | Anti-CaSR (ab19347) Abcam | Uterine Myometrium (low) Human term placenta | Relaxation? | Crankshaw et al., 2013 | |||
| PLACENTA | |||||||
| Mouse | Intra-placental yolk sac cells, parietal side + columnar side | Mouse anti-CaSR ADD | Intraplacental yolk sac cells, parietal side + columnar side Placental trophoblasts | Kovacs et al., 2002 | |||
| Human | Anti-CaSR Novus HL-1499 (WB) anti-CaSR Acris (IHC) | Term Placenta 130 kDa | Term villous STB Term villous cytotrophoblast Term extravillous trophoblast | Papadopoulou et al., 2014 | |||
| Human | First trimester villous STB | Monoclonal Anti-CaSR NPS Pharmaceuticals, human extracellular CaSR epitopes amino acids 214–235 or amino acids 374–391 | Villous STB (first trimester and term) Extravillous trophoblast (first trimester and term) | ↑ | Bradbury et al., 2002 | ||
| Human | Extravillous cytotrophoblast | Bradbury et al., 1998 | |||||