Table 1.
Isolates information, allelic profile, sequence types (ST), clonal groups (CG), and clonal complexes (CC) of strains studied.
| Serotype | Isolate | Source | Virulence profilea | Allelic profileb | STc | CGd | CC |
|---|---|---|---|---|---|---|---|
| O2:H5 | AM 166-1 | Adult cattle | stx2,saa,subA | 21,24,10,2,1,11,5 | 1179 | n.a. | Singleton |
| O5:NM | T 249-5 | Calf | stx1,eae,ehxA | 20,19,13,4,1,12,1 | 175 | 42 | 850 |
| O8:H16 | CM 15-2 | Ground beef | stx1,saa | 32,5,2,206,1,8,12 | 1066 | n.a. | Singleton |
| O8:H16 | FO 164 | Adult cattle | stx1,saa | 32,5,2,206,1,8,12 | 1066 | n.a. | Singleton |
| O8:H19 | 3M | Ground beef | stx1,stx2,ehxA | 4,5,2,111,1,5,1 | 379 | 63 | 230 |
| O8:H19 | 7M | Ground beef | stx2,ehxA | 7,5,77,4,1,13,23 | 1177 | n.a. | 230 |
| O15:H21 | FB 11 | Adult cattle | stx2 | 178,187,13,15,1,1,40 | 1156 | n.a. | Singleton |
| O20:H7 | AM 216-1 | Adult cattle | stx1,stx2 | 7,5,2,2,1,13,1 | 140 | 31 | 230 |
| O20:H19 | HT 1-6 | Hamburger | stx1,stx2,ehxA,saa,subA | 7,5,5,4,3,5,23 | 92 | 88 | 230 |
| O20:H19 | AP 28-1 | Adult cattle | stx2,ehxA,saa,subA | 179,5,5,4,3,5,23 | 1157 | n.a. | 230 |
| O20:HNT | T 250-4 | Calf | stx1,eae,ehxA | 10,188,46,39,1,5,5 | 1169 | n.a. | Singleton |
| O22:H8 | 36M | Ground beef | stx2 | 5,5,2,2,1,13,23 | 145 | 31 | 230 |
| O22:H8 | HW 1-15 | Hamburger | stx1,stx2,ehxA,saa | 5,5,2,2,1,13,23 | 145 | 31 | 230 |
| O25:H19 | FB 68 | Adult cattle | stx2,ehxA | 7,5,77,4,1,8,84 | 1170 | n.a. | Singleton |
| O26:H11 | T 246-6 | Calf | stx1,eae,ehxA | 7,8,65,4,1,5,275 | 1158 | n.a. | 106 |
| O26:H11 | T 8-1 | Calf | stx2,eae,ehxA | 7,8,2,4,122,5,250 | 943 | n.a. | Singleton |
| O38:H39 | T 246-1 | Calf | stx1,eae,ehxA | 11,190,231,208,19,112,263 | 1102 | n.a. | Singleton |
| O39:H49 | AM 169-3 | Adult cattle | stx1,stx2,ehxA,saa,subA | 7,5,2,101,1,5,1 | 1138 | n.a. | 230 |
| O39:H49 | AM 165-1 | Adult cattle | stx2,ehxA,saa,subA | 7,5,2,101,1,5,1 | 1138 | n.a. | 230 |
| O79:H19 | 27M | Ground beef | stx2,ehxA,saa,subA | 7,5,5,2,1,8,3 | 1171 | n.a. | None |
| O79:H19 | AM 168-3 | Adult cattle | stx2,ehxA,saa,subA | 7,5,5,2,1,8,3 | 1171 | n.a. | None |
| O88:H21 | HW 1-7 | Hamburger | stx1,stx2,ehxA,saa | 7,5,13,2,1,5,3 | 1172 | n.a. | Singleton |
| O91:H21 | HAB 14 | Hamburger | stx2,ehxA,saa | 5,5,5,2,3,5,5 | 89 | 34 | 230 |
| O91:H21 | AP 16-1 | Adult cattle | stx2,ehxA,saa | 5,5,5,2,3,5,5 | 89 | 34 | 230 |
| O103:NM | AM 202-2 | Adult cattle | stx2,eae,ehxA | 4,2,2,2,1,2,2 | 119 | 17 | 119 |
| O103:NM | T 46-6 | Calf | stx1,stx2,eae,ehxA | 4,2,2,2,1,2,2 | 119 | 17 | 119 |
| O103:H2 | FB 94 | Adult cattle | stx1,eae,ehxA | 4,2,2,2,1,2,2 | 119 | 17 | 119 |
| O111:NM | T 9-1 | Calf | stx1,eae,ehxA | 7,8,2,4,1,5,8 | 106 | 14 | 106 |
| O112:H2 | 2M | Ground beef | stx2 | 5,196,2,2,1,203,3 | 1159 | n.a. | Singleton |
| O113:NM | 15M | Ground beef | stx2 | 5,6,71,2,1,8,1 | 223 | 30 | 230 |
| O113:H21 | 5M | Ground beef | stx2,ehxA,saa,subA | 5,6,71,2,1,8,1 | 223 | 30 | 230 |
| O113:H21 | AP 97-3 | Adult cattle | stx2,ehxA,saa,subA | 5,6,71,2,1,8,1 | 223 | 30 | 230 |
| O116:H21 | CM 3-18 | Ground beef | stx2,ehxA,saa | 5,6,71,25,1,8,1 | 230 | 30 | 230 |
| O117:H7 | HT 2-2 | Hamburger | stx2 | 5,5,2,2,1,5,80 | 147 | 31 | 230 |
| O117:H7 | AP 32-1 | Adult cattle | stx2 | 5,5,2,2,1,5,80 | 147 | 31 | 230 |
| O118:H16 | T 250-2 | Calf | stx1,eae,ehxA | 7,8,2,2,1,5,8 | 1173 | n.a. | 106 |
| O120:H19 | FS 151 | Adult cattle | stx2,ehxA,saa | 7,5,5,2,1,8,3 | 1171 | n.a. | None |
| O141:H7 | AM 167-1 | Adult cattle | stx1,stx2,ehxA,saa,subA | 5,5,5,2,1,5,1 | 144 | 36 | 230 |
| O141:H8 | AP 31-1 | Adult cattle | stx1,stx2,ehxA,saa | 5,6,71,2,1,5,1 | 1174 | n.a. | 230 |
| O141:H8 | AP 21-1 | Adult cattle | stx2,ehxA,saa,subA | 5,5,5,2,1,5,1 | 144 | 36 | 230 |
| O145:NM | FB 5 | Adult cattle | stx2,eae,ehxA | 11,13,12,14,9,16,40 | 78 | 12 | 78 |
| O145:NM | AMB 166 | Adult cattle | stx2,eae,ehxA | 11,13,12,14,9,16,40 | 78 | 12 | 78 |
| O145:NM | FB 12 | Adult cattle | stx2,eae | 11,13,12,14,9,16,40 | 78 | 12 | 78 |
| O146:NM | FB 32 | Adult cattle | stx2,eae,ehxA | 11,13,12,14,9,16,40 | 78 | 12 | 78 |
| O157:H7 | FB 22 | Adult cattle | stx2,eae,ehxA | 1,1,4,3,2,4,4 | 66 | 11 | 66 |
| O157:H7 | GAL 26 | Human | stx2,eae,ehxA | 1,1,4,3,2,4,4 | 66 | 11 | 66 |
| O162:H7 | EN 1-4 | Hamburger | stx2 | 5,197,2,2,1,5,80 | 1160 | n.a. | 230 |
| O165:NM | T 83-1 | Calf | stx2,eae,ehxA | 68,19,244,39,1,17,94 | 1161 | n.a. | 253 |
| O171:HNT | 10M | Ground beef | stx2 | 4,6,2,2,1,2,5 | 1175 | n.a. | None |
| O171:H2 | CM 20-7 | Ground beef | stx2 | 4,6,2,40,1,2,5 | 130 | n.a. | None |
| O171:H2 | AM 217-1 | Adult cattle | stx2 | 4,6,2,40,1,2,5 | 130 | n.a. | None |
| O171:NM | 45M | Ground beef | stx2 | 4,6,2,40,1,2,5 | 130 | n.a. | None |
| O174:H21 | CM 25-12 | Ground beef | stx2 | 5,5,5,2,3,5,5 | 89 | 34 | 230 |
| O174:H21 | AM 170-3 | Adult cattle | stx1,stx2,ehxA,saa | 59,5,64,40,1,5,1 | 1178 | n.a. | 150 |
| O175:H8 | FB 40 | Adult cattle | stx2 | 5,5,2,2,1,5,23 | 146 | 31 | 230 |
| O177:NM | FO 127-3 | Adult cattle | stx2,eae,ehxA | 60,19,13,4,1,12,1 | 850 | n.a. | 850 |
| O178:H19 | FC 104 | Adult cattle | stx2 | 7,5,5,2,1,72,1 | 1176 | n.a. | Singleton |
| O178:H19 | 41M | Ground beef | stx2,ehxA,saa,subA | 179,5,5,4,3,5,23 | 1157 | n.a. | 230 |
| O185:H7 | CM 22-1 | Ground beef | stx2 | 5,5,2,2,1,5,80 | 147 | 31 | 230 |
a
Virulence profile. Genes taken into account: stx1, stx2, eae, ehxA, saa, subA.
b
Allelic profile based on MLST of 7 housekeeping genes (aspC, clpX, fadD, icdA, lysP, mdh, uidA). Boldface entries represent novel alleles.
c
Boldface entries indicate novel STs. These sequences were submitted to EcMLST with the name which figure in the column “isolate.”
d
n.a., not assigned.