Table 1.

Effect of different doses of affinity purified SP-HBP on sperm function and fertility tests during pre-freeze and frozen-thaw phases of cryopreservation

Sperm parameter	Pre-freeze				Frozen-thaw
	C1	10	20	40	C	10	20	40
Motility (%)	73.3±1.7a	76.7±1.7a	83.3± 1.7b	75.0±0.0a	45.0±2.9c	46.7±1.7c	58.3±4.4d	48.3±4.4 c
Viability (%)	68.7±3.6ab	61.2±1.2a	76.2±3.5a b	72.4±3.1 b	48.7±8.7c	47.8±6.2c	56.0±7.6c	52.4±8.2c
HOST (%)	35.3±2.4a	38.2±4.3a	42.1±4.1a	35.72±2.2a	35.0±2.2a	12.1±2.4b	18.6±3.9b	14.4±3.0b
Acrosome damage (%)
Cryo-Cap	22.9±2.6a	22.1±1.6a	23.3±1.5a	24.5±1.8a	29.2±2.9b	28.1±3.4b	28.4±1.7b	30.1±2.4b
Cryo-AR	12.1±4.5a	10.1±3.8a	10.5±4.4a	11.5±4.9a	16.3±4.3a	13.8±2.8a	14.6±3.7a	17.0±4.2a
In vitro capacitation/acrosome reaction (%)
Cap	-	-	-	-	39.1±3.4a	39.4±2.8a	44.4±3.0a	42.5±3.2a
AR	-	-	-	-	27.9±5.8a	30.1±6.1ab	34.0±5.1b	33.4±5.0 b
LPO (MDA, μM/109 spermatozoa)	14.7±5.7a	12.4±3.8a	11.9±3.6a	14.5±4.6a	50.3±2.8b	44.3±4.1bc	40.3±2.6c	45.3±4.9bc

HOST, hypo-osmotic swelling test; Cap, capacitated; AR, acrosome reacted; LPO, lipid peroxidation; MDA, malondialdehyde.

¹C, control (0), 10, 20, 40 (Dose of HBP in μg/mL).

Superscripts (a, b, ab, bc) indicate the difference at 5% level of significance within the rows.