Table 2.
Effect of affinity purified SP-HBP on sperm motility, viability and HOST (mean±SE) during pre-freeze and frozen-thaw phases of cryopreservation
| Bull number | Motility (%) | Viability | HOST | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
||||||||||
| Pre-freeze | Frozen-thawed | Pre-freeze | Frozen-thawed | Pre-freeze | Frozen-thawed | |||||||
|
|
|
|
|
|
|
|||||||
| C1 | T1 | C | T | C | T | C | T | C | T | C | T | |
| 1 | 70.0±0.0 | 80.0±0.0 | 58.3±3.4 | 66.6±2.9 | 73.0±6.6 | 79.6±4.5 | 59.5±2.2 | 64.8±2.7 | 30.6±3.7 | 36.2±3.3 | 18.1±3.2 | 25.1±4.9 |
| 2 | 70.0±2.9 | 78.3±3.3 | 55.0±2.9 | 61.7±1.7 | 69.1±7.5 | 76.9±5.8 | 54.9±7.1 | 61.6±6.0 | 32.5±8.1 | 54.6±2.5 | 16.9±5.9 | 27.8±4.8 |
| 3 | 68.3±4.4 | 73.3±4.4 | 50.0±5.8 | 56.6±4.4 | 65.9±3.8 | 71.0±3.6 | 50.4±8.8 | 56.4±6.1 | 36.1±8.0 | 46.3±6.2 | 27.5±7.5 | 35.3±5.7 |
| 4 | 61.7±1.7 | 70.0±2.9 | 45.0±2.9 | 50.0±2.9 | 61.3±2.1 | 67.0±1.0 | 43.0±7.2 | 47.2±7.2 | 25.3±5.1 | 30.1±3.3 | 17.7±1.7 | 20.7±2.1 |
| 5 | 63.3±3.3 | 68.3±3.3 | 41.7±4.4 | 43.3±3.3 | 55.7±2.8 | 59.3±3.3 | 38.2±3.1 | 44.9±0.2 | 16.0±0.7 | 22.0±2.6 | 8.8±4.6 | 12.6±3.2 |
| 6 | 65.0±2.9 | 71.7±1.7 | 46.7±6.0 | 51.7±6.0 | 65.1±0.5 | 69.3±1.8 | 48.5±1.4 | 59.5±0.7 | 21.6±4.6 | 32.5±4.0 | 12.6±2.0 | 24.6±1.5 |
| Combination factor mean | 66.4a | 73.6 b | 49.4c | 55.0d | 65.0a | 70.5a | 49.1b | 55.7b | 27.0a | 36.9b | 16.9c | 24.3a |
SP-HBP, seminal plasma-heparin binding protein; HOST, hypo-osmotic swelling test; SE, standard error.
1
C, control without HBP; T, supplemented with SP-HBP.
Superscripts a, b, c, d indicate the difference at 5% level of significance within the columns.