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Histological analysis. (a) In hematoxylin and eosin staining, most of the defect areas were filled with fibrous connective tissue, and newly formed bone tissue had a woven, trabecular appearance. (b) Masson's trichrome staining revealed abundant collagenous tissue around the regenerated tissue. Vasculatures were observed inside and around the new bone. Asterisks and arrows indicate bone tissue and vasculatures, respectively. Scale bars = ((a), (b)(A)) 200μm, ((b)(B)) 15μm.
