Table 1.
Primers sequences used for quantitative reverse transcription PCR.
| Target gene | Primer sequence (5′-3′) | |
|---|---|---|
| RUNX2 | Forward | TGTCATGGCGGGTAACGAT |
| Reverse | TCCGGCCCACAAATCTCA | |
|
| ||
| ALP | Forward | TCCGAGATGGTGGAAATAGC |
| Reverse | GGGCCAGACCAAAGATAGAG | |
|
| ||
| Osteopontin | Forward | GATGATGGAGACGATGTGGATA |
| Reverse | TGGAATGTCAGTGGGAAAATC | |
|
| ||
| Osteocalcin | Forward | CTGGTCCAGCAGATGCAAAG |
| Reverse | GGTCAGCCAGCTCGTCACAGTT | |
|
| ||
| BMP7 | Forward | TCGTGGAGCATGACAAAGAG |
| Reverse | GCTCCCGAATGTAGTCCTTG | |
|
| ||
| AXIN | Forward | ACGGATTCAGGCAGATGAAC |
| Reverse | CTCAGTCTGTGCCTGGTCAA | |
|
| ||
| β-catenin | Forward | TACTGAGCCTGCCATCTGTG |
| Reverse | ACGCAGAGGTGCATGATTTG | |
|
| ||
| VEGF | Forward | CTATGGCAGGAGGAGAGCAC |
| Reverse | GCTGCAGGAAACTCATCTCC | |
|
| ||
| GAPDH | Forward | CATTGCCCTCAATGACCACT |
| Reverse | TCCTTGGAGGCCATGTAGAC | |