Figure 8.

OrexinA increased firing ex vivo in medial NAc Shell neurons from alcohol-drinking mice. (A) Example showing that 100 nM orexinA (OxA) increased firing in the mNAsh neurons from adult alcohol-drinking mice; firing was evoked by 500-ms depolarizing current pulses (See Section Methods). Example is from rheobase, the minimum current required to evoke firing, in this neuron (B,C) Averaged data showing the increased number of action potentials generated with OxA (B) at rheobase, and (C) at the current step above rheobase, and that blocking OX1Rs with SB prevented the OxA increase in firing. ^*p < 0.05.