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miR-186 regulates cell cycle regulatory proteins about G1/S transition. A. Real time RT-PCR determined p21, p27 and Cyclin D1 in PC3 and DU145 with miR-186 overexpression of knockdown. B. Western blot determined p21, p27, Cyclin D1, Rb and p-Rb level in indicated cells with miR-186 overexpression of knockdown. α-Tubulin was used as the lading control. Error bars represent ± S.D. *P < 0.05.
