Figure 6.

Confirming PCDH20 is required for 5-FU-induced apoptosis. (A) PCDH20 was ectopically expressed in vector- and miR-122-expressing cells. Western blot analysis was performed to confirm the rescued expression of PCDH20. As a result, Akt and mTOR phosphorylation were inhibited. Cells were treated with increasing concentrations of 5-FU. MTT assays (B) and DNA fragmentation assays (C) showed that restoration of PCDH20 expression re-sensitized miR-122-expressing cells to 5-FU treatment. The data are presented as the mean ± S.D. of triplicate experiments.