Skip to main content
. 2016 Aug 1;6(8):1741–1757.

Figure 2.

Figure 2

DEC2 significantly suppresses cell proliferation and promotes apoptosis in GC cell lines. A-C: DEC2 expression as shown by western blot and qRT-PCR in normal gastric cell line and GC cell lines. D-F: DEC2 expression in MGC803 and MKN-45 cells transfected with DEC2 vector and empty vector (control) assessed by western blot and qRT-PCR. G, H: Cell viability of MGC803 and MKN-45 cells were measured by CCK8 assay. I: Comparison of proliferation ratio of MGC803 and MKN-45 cells transfected with DEC2 vector and empty vector by EdU incorporation assay. J. The expressions of apoptotic-related proteins, bcl-2, Bax, and survivin, were detected in GC cells using western blot. K. GC cells transfected with DEC2 were stained with Annexin V-FITC/PI and analyzed by flow cytometry. The percentage of apoptotic cells is shown in Supplementary Figure 1C. The error bars indicate ± SEM. *P<0.05; **P<0.01 by Student’s t-test. All the results were repeated thrice.