DEC2 suppresses GC cell proliferation and metastasis in vivo. A, B, I, J. Tumor volume. C, K. Weight are shown after subcutaneous injection with DEC2/MKN-45, DEC2-sh/HGC27 and their corresponding control cells, respectively. D, L. Lung metastasis in mice after intravenous injection with DEC2/MKN-45, DEC2-sh/HGC27 and their corresponding control cells by H&E staining. E, M. The number of tumor macrometastatic nodules in the lungs of mice after intravenous injection with DEC2/MKN-45, DEC2-sh/HGC27 and their corresponding control cells, respectively. F. Expression of DEC2 and E-cadherin in metastasis nodules of DEC2/MKN-45-treated mice lungs were determined by immunohistochemistry. G, H. DEC2, E-cadherin, bcl-2, and Bax expression were determined in three pairs of xenograft tumors of group NC and group DEC2 overexpression by western blot. “C” means group NC, “D” means group DEC2 overexpression. n = 6. Quantitative results are shown in the right panel. The error bars indicate ± SEM. *P<0.05; **P<0.01 by Student’s t-test.