Figure 5.
DEC2 inhibits EMT in gastric cancer cells. A. The morphological changes of MGC803, MKN-45 and HGC27 cells after up or down regulation of DEC2 under light microscope (magnification ×100). B. Immunofluorescence staining results of epithelial marker E-cadherin and mesenchymal marker vimentin in stable transfected DEC2/MGC803, DEC2/MKN-45, DEC2-sh/HGC27 cells and their corresponding control cells (magnification ×400). C. Comparison of EMT-related proteins in stable transfected DEC2/MGC803, DEC2/MKN-45, DEC2-sh/HGC27 cells and their corresponding control cells. Quantitative results are shown in the bottom panel. The error bars indicate ± SEM. *P<0.05; **P<0.01 by Student’s t-test. All the results were repeated thrice.