DEC2 inhibits EMT through inactivation of ERK/NF-κB/E-cadherin pathway. A, D. Western blot results of NF-κB p65, p-ERK, and E-cadherin expression levels in DEC2/MGC803, DEC2/MKN-45, DEC2-sh/HGC27 and their corresponding control cells. Quantitative results are shown in Supplementary Figure 1. B, E. Western blot results of NF-κB p65, p-ERK, and E-cadherin expression levels in DEC2/MGC803, DEC2/MKN-45, DEC2-sh/HGC27 and their corresponding control cells after treated with ERK inhibitor U0126 (10 μM). C. Comparisons of the migration and invasion ability in stable transfected DEC2/MGC803 and DEC2/MKN-45 cells after treated with ERK inhibitor U0126 (10 μM) or not. Quantitative results are shown in the right panel. F. Comparisons of the migration and invasion ability in DEC2-sh/HGC27 and their corresponding control cells after treated with ERK inhibitor U0126 (10 μM) or not. Quantitative results are shown in the bottom panel. The error bars indicate ± SEM. *P<0.05; **P<0.01 by Student’s t-test. All the results were repeated thrice.