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. 2016 Aug 1;6(8):1758–1771.

Figure 4.

Figure 4

Cordycepin-mediated ERK inhibition downregulates DUSP5 and phosphorylates JNK. A: Representative western blots of TK-10 cells treated with cordycepin showing the expression of p-ERK, ERK, DUSP5, p-JNK, JNK. TK-10 cells were incubated with siRNA directed against ERK (si-ERK) or negative control siRNA for 48 h, and transfected with an ERK-overexpressing construct for 48 h. B: Cordycepin-mediated DUSP5 inhibition phosphorylates JNK and increases Bax. TK-10 cells were incubated with siRNA directed against DUSP5 (si-DUSP5) for 48 h, and transfected with a DUSP5-overexpressing construct for 48 h. TK-10 cells were treated with cordycepin or cordycepin and 10 mM SP600125 for 48 h. Bar graphs represent densitometric analysis of at least three separate experiments (means ± SD). *P<0.05, **P<0.01, and ***P<0.001.