Table 1.
Primer sequences, amplicon size, and annealing temperatures for MST1R [GenBank: NM_002447] bisulfite sequencing (BSP), quantitative methylation specific PCR (QMSP) and expression
| Primer set | Sense primer sequence (5’-3’) | Antisense primer sequence (5’-3’) | Amplicon size (bp) | Location [bp upstream (up) or downstream (down) TSS] | Annealing temp (°C) |
|---|---|---|---|---|---|
| BSP | |||||
| MST1R_B_1 | GTTATTGAGGGTGTTGTTATTAAGTG | ACCTAACCCAAACCCTCC | 264 | 612 up to 348 up | 60 |
| MST1R_B_2 | AGGTGAAGGTATAGGAGTTAGG | AAATTCCTATAAAACCCAAATC | 272 | 417 up to 145 up | 60 |
| MST1R_B_3 | GGTAGGGATTTTTTAGGGTTT | CACCATAACCTATACCAAACCTC | 210 | 33 up to 177 down | 60 |
| QMSP | |||||
| MST1R up | TTAAGGGTCGGAAGAGTC | ATACACTAACGCTTAACGCTC | 128 | 540 up to 412 up | 60 |
| MST1R TSS | AGCGTTAGTGTATAGCGGC | TAAACAACGATCCCGACA | 169 | 270 up to 101 up | 60 |
| Expression | |||||
| MST1R total | GGCTGAGGTCAAGGATGTGCT | GCCTTTGCCAATGACTCGGT | 73 | - | 62 |
| MST1R long | CTCTGGGGACCAGGTTTTC | ATGAAATGCCATGCCCTTAGa | 93 | - | 62 |
| NF-κB | GCTTAGGAGGGAGAGCCCT | CTGCCATTCTGAAGCCGGG | 86 | - | 61 |
a
Primer sequence from [23].