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. 2016 Aug 30;6:32411. doi: 10.1038/srep32411

Table 1. Bacterial richness and diversity index for 16S region libraries in the soil from each site after fire.

Years after fire No. of Sequencesa OTUs coverage (%) Normalizationb
Observed OTUs Estimated OTUs Shannon Shannon evenness
2 39762 ± 3799 0.98 1641 ± 34c 2369 ± 54c 5.2 ± 0.2c 0.69 ± 0.03c
60 32269 ± 1974 0.98 1524 ± 124d 2027 ± 138d 5.3 ± 0.1c 0.72 ± 0.01c
152 32069 ± 4704 0.99 1195 ± 112e 1728 ± 153e 4.8 ± 0.2c 0.67 ± 0.01c

aAverage number of sequence obtained from the three replicates in each site with standard deviation.

bData were calculated at 3% genetic distance level with standard deviation based on the same number of sequences from each replicate (27699/sample) in Mothur. OTUs containing singleton sequence were discarded.

Different letters (c, d and e) in each column represent significant difference level at 0.05 between each site.