Table 1. Bacterial richness and diversity index for 16S region libraries in the soil from each site after fire.
Years after fire | No. of Sequencesa | OTUs coverage (%) | Normalizationb |
|||
---|---|---|---|---|---|---|
Observed OTUs | Estimated OTUs | Shannon | Shannon evenness | |||
2 | 39762 ± 3799 | 0.98 | 1641 ± 34c | 2369 ± 54c | 5.2 ± 0.2c | 0.69 ± 0.03c |
60 | 32269 ± 1974 | 0.98 | 1524 ± 124d | 2027 ± 138d | 5.3 ± 0.1c | 0.72 ± 0.01c |
152 | 32069 ± 4704 | 0.99 | 1195 ± 112e | 1728 ± 153e | 4.8 ± 0.2c | 0.67 ± 0.01c |
aAverage number of sequence obtained from the three replicates in each site with standard deviation.
bData were calculated at 3% genetic distance level with standard deviation based on the same number of sequences from each replicate (27699/sample) in Mothur. OTUs containing singleton sequence were discarded.
Different letters (c, d and e) in each column represent significant difference level at 0.05 between each site.