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. 2016 Aug 30;6:32184. doi: 10.1038/srep32184

Figure 4. The attachment, proliferation, survival, and secretion of neurotrophic factors of ASCs on cryoGelMA NGCs in vitro.

Figure 4

(A) Live/dead analysis of ASCs on the NGCs after 2 days of cell seeding (Scale bars = 200 μm, scale bars in upper left inset = 25 μm). (B) Staining of F-actin by rhodamine phalloidin. The ASCs spreaded on the surface of NGCs after 2 days of culture (Scale bars = 200 μm, scale bars in upper left inset = 25 μm). SEM micrographs of ASCs cultured on the TCP (C) and NGCs (D). (E) Analysis of the proliferation of ASCs on the TCP and NGCs after 1, 2, and 3 days of culture (n = 3). (F) Gene expression of major neurotrophic factors (NGF, BDNF, and GDNF) of ASCs on the TCP and NGCs at 2 days post-seeding. *p < 0.05 for comparison with the TCP.