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. 2016 Aug 30;6:31750. doi: 10.1038/srep31750

Figure 6. PEG chain length in DSPE-PEG micelles affects cholesterol reduction in Npc1−/− cells.

Figure 6

(A) Npc1−/− MEFs were treated with DSPE-PEG micelles (10 μM) with increasing PEG chain length in the presence or absence of low dose HPβCD (0.1 mM). Cells were washed and stained for filipin. (A) Longer PEG chain lengths (>2,000 MW) show effective cholesterol reduction when treated with 10 μM. Micelle treatment with 0.1 mM HPBCD additionally reduces cholesterol when administered with DSPE-PEG micelles. (B) DLS was used to calculate average diameter of DSPE-PEG micelles, data is reported as distributed by number. Results were tested for statistical significance using one-way ANOVA with Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001 and NS = not significant.