Table 2. Detection of PrPSc by protein-misfolding cyclic amplification in biological samples after p.o. administration in fasted mice.
| PMCA Round | 3 hr after P.O. |
24 hr after P.O. |
||||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
| Stomach | 4/4 (100%) | 4/4 (100%) | 4/4 (100%) | 4/4 (100%) | 2/4 (50%) | 3/4 (75%) | 4/4 (100%) | 4/4 (100%) |
| Duodenum | 2/4 (50%) | 4/4 (100%) | 4/4 (100%) | 4/4 (100%) | 1/4 (25%) | 2/4 (50%) | 3/4 (75%) | 3/4 (75%) |
| Jejunum | 1/4 (25%) | 3/4 (75%) | 4/4 (100%) | 4/4 (100%) | 2/4 (50%) | 3/4 (75%) | 4/4 (100%) | 4/4 (100%) |
| Ileum | 1/4 (25%) | 4/4 (100%) | 4/4 (100%) | 4/4 (100%) | 2/4 (50%) | 3/4 (75%) | 4/4 (100%) | 4/4 (100%) |
| Colon | 0/4 (0%) | 3/4 (75%) | 3/4 (75%) | 3/4 (75%) | 0/4 (0%) | 2/4 (50%) | 4/4 (100%) | 4/4 (100%) |
| Spleen | 0/4 (0%) | 1/4 (25%) | 2/4 (50%) | 3/4 (75%) | 0/4 (0%) | 0/4 (0%) | 1/4 (25%) | 1/4 (25%) |
| Liver | 0/4 (0%) | 4/4 (100%) | 4/4 (100%) | 4/4 (100%) | 1/4 (25%) | 1/4 (25%) | 2/4 (50%) | 3/4 (75%) |
| Kidney | 0/4 (0%) | 0/4 (0%) | 3/4 (75%) | 4/4 (100%) | 0/4 (0%) | 0/4 (0%) | 0/4 (0%) | 1/4 (25%) |
| Whole blood | 0/4 (0%) | 1/4 (25%) | 1/4 (25%) | 1/4 (25%) | 0/4 (0%) | 0/4 (0%) | 0/4 (0%) | 0/4 (0%) |
| Plasma | 0/4 (0%) | 0/4 (0%) | 0/4 (0%) | 1/4 (25%) | 0/4 (0%) | 0/4 (0%) | 0/4 (0%) | 0/4 (0%) |
Protein-misfolding cyclic amplification (PMCA) was performed with biological samples obtained at 3 and 24 hr after p.o. administration of 127I-PrPSc in fasted (16 hr) mice. Four separate sets of samples were tested and values were presented as the number of PMCA positive samples over four determinations. The percentage of PMCA positive samples were also reported in parenthesis. Representative blotting results of PMCA reaction are shown in Fig. S2.