Table 2. IC50 values for rAtPDF2.3 against S. cerevisiae wild type (WT) and knockout mutants.
| Microorganism | IC50 (μM) | P-value |
|---|---|---|
| Saccharomyces cerevisiae BY4741 WT | 8.1 ± 0.9 | NA |
| Saccharomyces cerevisiae BY4743 WT | 3.3 ± 0.0 | NA |
| Saccharomyces cerevisiae BY4741 ∆trk1 | 1.5 ± 0.0 | <0.0001 |
| Saccharomyces cerevisiae BY4741 ∆trk2 | 7.0 ± 0.6 | 0.5862 |
| Saccharomyces cerevisiae BY4741 ∆hal5 | 4.0 ± 0.4 | <0.0001 |
| Saccharomyces cerevisiae BY4741 ∆prm6 | 7.6 ± 0.2 | 0.9958 |
| Saccharomyces cerevisiae BY4741 ∆arl1 | 5.7 ± 0.2 | 0.0033 |
| Saccharomyces cerevisiae BY4741 ∆qdr2 | 2.9 ± 0.2 | <0.0001 |
| Saccharomyces cerevisiae BY4741 ∆sat4 | 5.1 ± 0.1 | 0.0003 |
| Saccharomyces cerevisiae BY4741 ∆vhc1 | 8.0 ± 0.2 | >0.9999 |
| Saccharomyces cerevisiae BY4741 ∆ppz2 | 8.6 ± 0.5 | 0.9868 |
| Saccharomyces cerevisiae BY4741 ∆tok1 | 7.7 ± 0.5 | 0.9995 |
| Saccharomyces cerevisiae BY4741 ∆nha1 | 9.1 ± 0.7 | 0.2161 |
| Saccharomyces cerevisiae BY4741 ∆ppz1 | 10.1 ± 0.5 | 0.0120 |
| Saccharomyces cerevisiae BY4741 ∆kch1 | 8.9 ± 0.3 | 0.8144 |
| Saccharomyces cerevisiae BY4741 ∆kkq8 | 7.1 ± 0.1 | 0.7009 |
| Saccharomyces cerevisiae BY4743 ∆frq1 | 3.1 ± 0.1 | 0.0935 |
WT and knockout yeast strains were treated with a concentration range of rAtPDF2.3 for 24 hours, after which the IC50 values were determined spectrophotometrically; IC50, the concentration required for 50% growth inhibition as compared to control treatment. Mean ± SEM is shown of at least three independent experiments (n ≥ 3). ANOVA followed by Dunnett post hoc test was performed to analyse significant differences between the effect of rAtPDF2.3 on BY4741 WT yeast and knockout mutants; the adjusted P-values are presented. Unpaired Student’s t-test was performed to analyse significant differences between the effect of rAtPDF2.3 on BY4743 WT and the FRQ1 knockout mutant. NA, not applicable.