Table 3. IC50 values for rHsAFP1 against S. cerevisiae wild type (WT) and knockout mutants.
| Microorganism | IC50 (μM) | P-value |
|---|---|---|
| Saccharomyces cerevisiae BY4741 WT | 4.8 ± 0.1 | NA |
| Saccharomyces cerevisiae BY4741 ∆trk1 | 2.8 ± 0.1 | <0.0001 |
| Saccharomyces cerevisiae BY4741 ∆hal5 | 4.6 ± 0.0 | 0.1073 |
| Saccharomyces cerevisiae BY4741 ∆arl1 | 5.2 ± 0.1 | 0.0281 |
| Saccharomyces cerevisiae BY4741 ∆qdr2 | 4.8 ± 0.0 | 0.4534 |
| Saccharomyces cerevisiae BY4741 ∆sat4 | 4.7 ± 0.1 | 0.1865 |
WT and knockout yeast strains were treated with a concentration range of rHsAFP1 for 24 hours, after which the IC50 values were determined spectrophotometrically; IC50, the concentration required for 50% growth inhibition as compared to control treatment. Mean ± SEM is shown of at least three independent experiments (n ≥ 3). ANOVA followed by Dunnett post hoc test was performed to analyse significant differences between the effect of rHsAFP1 on WT yeast and knockout mutants; the adjusted P-values are presented. NA, not applicable.