Figure 6. Overexpression of miR-195-5p inhibited the activation of Notch signaling pathway and is associated with severe cytopenias, bone marrow hypocellularity and superior survival.

(A) Overexpression of miR-195-5p downregulated the expression of Notch signaling-related proteins, including DLL1 and cleaved Notch1. Overexpression of miR-195-5p increased the expression of the apoptosis-related protein BAX and decreased the expression of proliferation-related proteins bcl-2. (B) The number of peripheral cytopenias exhibited positive correlation with miR-195-5p expression in MDS patients (Spearman r = 0.376, P = 0.026). (C) The patients with bone marrow hypocellularity also exhibited reduced expression of miR-195-5p compared to patients with hypercellularity. (D) The patients with high expression of miR-195-5p exhibited relatively superior overall survival compared with patients with a low expression of miR-195-5p, but no significant difference was observed. A representative Western blot graphic is shown from three independent experiments. Error bars throughout represent the s.e.m. Correlation coefficient was calculated using Spearman analysis. In comparison analysis, unpaired Student’s t tests were used to calculate the P value. Statistical significance in survival analysis was determined using the log-rank test.