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. 2016 Aug 30;6:32259. doi: 10.1038/srep32259

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Copyright © 2016, The Author(s)

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(A) Schematic illustration of indirect coculture between RAW 264.7 cells and MCF7 cells. ϕ 1μm cell culture filter insert was used. TRAP staining of RAW 264.7 cells coculture with MCF7 cells in the presence of RANKL (B), coculture with MCF7 cells in the presence of RANKL and IFN-γ (C), and coculture with ADAM17-knockeddown MCF7 cells in the presence of RANKL and IFN-γ (D) are shown, respectively. Green arrowhead indicates TRAP+ multinucleated cells. (E) The number of TRAP+ multinucleated cells in each group are shown. *p < 0.05 vs coculture with ADAM17-knockeddown MCF7 cells in the presence of RANKL and IFN-γ. NS: no significant difference between the groups. Real-time PCR analysis for osteoclast marker gene expression. Fold change from the control of TRAP (F), ATP6v0d2 (G), and cathepsin K (H) were shown, respectively. *p < 0.05 VS coculture with ADAM17-knockeddown MCF7 cells in the presence of RANKL and IFN-γ.