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. 2016 Aug 30;6:32068. doi: 10.1038/srep32068

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Optical coherence tomography (OCT) showing 3D reconstructions of EHTs (first row) formed by stacking serial cross-sectional images (second row) (scale bar, 1 mm). (b) OCT was used to follow EHT thickness and width between 3 and 16 days post-seeding. (c) Mean cross-sectional area assessed in 9-, 16-, and 23-day old EHTs. (d) Fluorescent staining for actin filaments (phalloidin, red) and nuclei (DAPI, blue) show the formation of NRVMs into rod-shaped cells within the scaffold after 16 days of culture. Striations indicate the formation of sarcomeres (scale bar, 25 μm). (e) Staining for cardiac Troponin T (green) and Connexin 43 (red) of an EHT seeded with human embryonic stem cell-derived cardiomyocytes. Note the formation of gap junctions between neighboring cardiomyocytes after 16 days in culture (scale bar, 25 μm).