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. 2016 Aug 30;6:32268. doi: 10.1038/srep32268

Figure 2. MiR-98 and DNA methylation level in the placental tissues from patients with GDM.

Figure 2

The miR-98 level was detected in JEG-3 cells treated with 5-aza by qRT-PCR (A). U6 serves as an internal reference to normalize the experimental error. Global DNA methylation level in miR-98 mimic or inhibitor-treated cells with or without 5-aza was detected by immunohistochemistry and estimated by the content of global 5-meC (B). The stain was developed with DAB and cell nuclei were stained with haematoxylin. Brown indicates the positive signals. Scale bar = 50 μm. The histogram represents the ratio of 5-meC positive cells (%). *P < 0.05; **P < 0.01.