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. 2016 Aug 30;6:32268. doi: 10.1038/srep32268

Figure 3. The prediction of the miR-98 target gene.

Figure 3

MiR-98 binding site in the 3′-UTR region of Mecp2 was conserved in cross-species (A). 5-aza treatment enhanced Mecp2 mRNA level and protein level detected by qRT-PCR (B) and western blot (C). Gapdh and β-ACTIN serve as an internal reference for qRT-PCR and western blot, respectively. For western blot, the gels had been run under the same experimental conditions. The bands were analyzed using Quantity One analyzing system (Bio-Rad, Hercules, CA, USA). The histogram represents the optical densities of the signals quantified by densitometric analysis and expressed as MECP2 intensity/β-ACTIN intensity to normalize for gel loading and transfer. *P < 0.05; **P < 0.01.