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. 2016 Aug 30;6:32268. doi: 10.1038/srep32268

Figure 4. The confirmation of the miR-98 target.

Figure 4

(A) The effects of miR-98 expression dysregulation on Mecp2 translation. HEK-293T cells were co-transfected with mimic control, miR-98 mimic, inhibitor control or miR-98 inhibitor and Mecp2-pGL3 for dual-luciferase assay. (B) Mutation analysis of the miR-98 binding site. Mutating the binding site of miR-98 in the 3′-UTR of Mecp2 was used as control (Mecp2-pGL3-Mut). PRL-TK containing Renilla luciferase was co-transfected for data normalization.*P < 0.05, **P < 0.01.