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. 2016 Aug 30;6:32268. doi: 10.1038/srep32268

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The Trpc3 expression in the placental tissues from patients with GDM was detected by qRT-PCR (A) and western blot (B). The gray histograms of B1–B4 represent the protein level of MECP2 in placental tissues in Y < 25, Y25~30, Y30~35 and Y > 35 age group, respectively. Gapdh and β-ACTIN serve as an internal reference for qRT-PCR and western blot, respectively. *P < 0.05.