Table 2. Primer sequences.

Gene Name	Primer Sequence	Accession Number	Size and Location	Application
MeCP2 3′-UTR	Forward/SpeI:5′-GACTAGTCCCACCCTTGTTCCAGTTGT-3′	NM_004992.3	365 bp (5105–5469)	PCR
	Reverse/XbaI:5′-GCTCTAGATGCCACCCAACAGAAGATT-3′
MeCP2 3′-UTR (mutation)	Forward:5′-GTTCCAGTTGTTAGTTACTATATCCTCTCCTGACAATACT-3′	NM_004992.3	365 bp (5105–5469)	PCR
	Reverse:5′-TATAGTAACTAACAACTGGAACAAGGGTGGG-3′
MeCP2 (coding region)	Forward/kozak-BamHI: 5′-CGCGGATCGCCACCATGGTAGCTGGGATGTTAGGGCT-3′	NM_004992.3	1461 bp (227–1687)	RT-PCR
	Reverse/EcoRI: 5′-CCGGAATTCTCAGCTAACTCTCTCGGTCACGG-3′
MeCP2	Forward: 5′-TGCAAAGAGGAGAAGATGCCCAGA-3′	NM_004992.3	169 bp (1463–1631)	qRT-PCR
	Reverse: 5′-GCCTTGGCATGGAGGATGAAACAA-3′
Trpc3	Forward: 5′-CATTCCTGGCCATTGGCTACT-3′	NM_001130698.1	117 bp (1375–1491 bp)	qRT-PCR
	Reverse: 5′-GCAGACCCAGGAAGATGATGAA-3′
Gapdh	Forward: 5′-TGGTATCGTGGAAGGACTCA-3′	NM_002046.4	129 bp (678–806)	qRT-PCR
	Reverse: 5′-GTAGAGGCAGGGATGATGTTC-3′