Figure 5.

The Pten PDZ-BD acts to suppress tumorigenesis. (a) Spontaneous tumour incidence in 16-month-old mice. (b) Spectrum of spontaneous tumour types. (c) Representative images and histological analysis of spontaneous tumours in mutant mice. (d) Paraffin section of a mesenteric lymphoma immunostained for B220 and CD3. (e) Interphase FISH for chromosomes 4 and 7 on single-cell suspensions of lymphomas from four independent Pten^ΔTKV/ΔTKV mice. Normal spleens from WT mice were used as controls. n, number of samples from independent mice. (f) Western blot analysis of tumour lysates probed with the indicated antibodies. Ponceau S staining of blotted proteins served as a loading control. (g) Survival curves for Eµ–Myc and Pten^ΔTKV/ΔTKV; Eµ–Myc mice dying of lymphomas. (h) Lung tumour burden of Kras^LA1 and Pten^ΔTKV/ΔTKV; Kras^LA1 mice. (i) Lung tumour incidence of DMBA-treated mice. (j) Average number of lung tumours in DMBA-treated mice. n in a,g–i represents the number of mice. Statistical significance was determined by Chi-square test in a,b,i, unpaired t-test in e, log-rank test in g, and non-parametric Mann Whitney test in h,j. All data presented in bar graphs represents mean ± s.e.m. *P <0.05, **P < 0.01, ***P < 0.001. Unprocessed original scans of blots can be found in Supplementary Fig. 6 and statistics source data in Supplementary Table 1.