Table 1.
Bacterial strains and plasmids
| Name | Description | Reference |
|---|---|---|
| Plasmids | ||
| pETDuet-1 | pBR322 ori with PT7; AmpR | Novagen |
| pCDFDuet-1 | CDF ori with PT7; SmR | Novagen |
| pET-A | pETDuet-1 carrying aibA, aibB, aibC and liuC | This study |
| pCDF-E | pCDFDuet-1 carrying ERG10 and ERG13 | This study |
| pCDF-EV1 | pCDFDuet-1 carrying ERG10, ERG13 and HlVPS syn | This study |
| pCDF-EV2 | pCDFDuet-1 carrying ERG10, ERG13 and FvCHS2-1 syn | This study |
| pCDF-EV3 | pCDFDuet-1 carrying ERG10, ERG13 and HpCHS | This study |
| Strains | ||
| Myxobacteria | Myxococcus xanthus (CGMCC 1.3865) | CGMCC |
| Yeast | Saccharomyces cerevisiae BY4742 | 29 |
| APG-0 | E. coli BL21 (DE3) with pETDuet-1 and pCDFDuet-1 | This study |
| APG-IV | E. coli BL21 (DE3) with pET-A and pCDF-E | This study |
| APG-1 | E. coli BL21 (DE3) with pET-A and pCDF-EV1 | This study |
| APG-2 | E. coli BL21 (DE3) with pET-A and pCDF-EV2 | This study |
| APG-3 | E. coli BL21 (DE3) with pET-A and pCDF-EV3 | This study |
CGMCC China general microbiological culture collection center