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. 2016 Aug 30;17(1):20. doi: 10.1186/s12867-016-0074-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Polymerase stress increases transcription of the long RPB2 mRNA. a Northern analysis [29] of mRNA levels when shifting the rpb1-1 mutant to the non-permissive temperature of 37 °C. Northern blot analysis of mRNA levels in the b RNAPII anchor-away cells (RPB1-FRB; MVY859) and c Tbp1 anchor-away cells (TBP1-FRB; MVY868) after addition of rapamycin to deplete FRB tagged proteins from the nucleus. The long and short isoform of the RPB2 mRNA are indicated