Fig. 2.

Chromatin Immunoprecipitation of RNA polymerase subunits at the RPB2 locus after RNAPII depletion. a The schematic indicates locations of the primers used for chromatin immunoprecipitation at the 5′ and 3′ end of the RPB2 locus. IP was performed using the b anti-CTD antibody for RNAPII. c anti-FLAG antibody for the Rpa190-FLAG subunit of RNAPI and d anti-Rpc34. for RNAPIII. RDN37 and tRNA phe are known targets of RNAPI and RNAPIII respectively and thus were used as positive controls