Table 1.
Yeast strains used in this study
| Strain | Original name, genotype (annotation) | Notes | References |
|---|---|---|---|
| MVY150 | W303-1B, MATα ade2-1 trp1-1 can1-100 leu2-3, 112 his3-11,15 ura3-1 | [7] | |
| MVY451 | SYY9, MATa ade2-1 his3-11, 15 leu2-3, 112 trp1-1 ura3-1 can1-100 rpb1-1 (derived from W303) | [27] | |
| MVY845 | NOY408-1a, MATα rpa135::LEU2 ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 carrying pNOY102 (GAL7-35S rDNA URA3) | [28] | |
| MVY851 | MATα rpa135::LEU2 ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 carrying pNOY102 (GAL7-35S rDNA URA3) rpb1-1 | This study | |
| MVY858 | HHYy168, MATα tor1-1 fpr1::NAT RPL13A-2xFKBP12::TRP1 | [11] | |
| MVY859 | HHY170, MATα tor1-1 fpr1::NAT RPL13A-2xFKBP12::TRP1 RPO21-FRB::KANMX6 | [11] | |
| MVY860 | MVY858 with RPA190-FRB::KANMX6 | This study | |
| MVY862 | MVY858 with RPC160-FRB::KANMX6 | This study | |
| MVY868 | MVY858 with TBP1-FRB::KANMX6 | This study | |
| MVY872 | MVY859 with RPA190-FRB-HIS3MX | This study | |
| MVY874 | MVY859 with RPC160-FRB-HIS3MX | This study | |
| MVY879 | MVY872 with RPC160-FRB-LEU2 | This study | |
| CY1940 | MVY858 with RPA190 FLAG | Pol I AA | This study |
| CY2032 | MVY859 with RPA190 FLAG | Pol I AA Pol II AA | This study |