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Schematic diagram showing the construction of plasmid pGL. Arrows indicate the locations and orientations of open reading frames. The adapter used for adding multiple-cloning sites (MCS) and the restriction enzyme sites were shown. The GFP fragment was obtained from EcoR I digestion of pACL2084. BLA, ampicillin resistance gene; EM-R, erythromycin resistance gene; CAT, chloramphenicol resistance gene.
