Figure 5.

LXR agonist treatment increases eosinophilic airway inflammation and type 2 cytokine production in house dust mite (HDM)‐induced asthma. (A) mRNA expression levels of LXR target genes in the lungs 6 h after carrier or GW3965 treatment by oral gavage. (B) Wild‐type mice were sensitized against HDM and subsequently challenged intranasally with HDM for 4 days. Two hours before intranasal HDM challenge, mice were treated with carrier or GW3965 administered by oral gavage. Differential cell counts in the bronchoalveolar lavage were measured by flow cytometry. (C) Mediastinal lymph nodes were isolated and cells were restimulated with HDM for 48 h. Cytokine levels were measured by luminex analysis. Statistical analysis between two groups was performed using a Mann–Whitney U test. Data are shown as mean ± SD, n = 7–10 mice per group. The results show one representative experiment out of two independent experiments. Significant P‐values were ranked as P < 0.05 (*), P < 0.01 (**), and P < 0.001 (***).