Figure 2.
Channel voltage dependence in the cell-excised inside-out configuration. Experiments were performed under symmetrical NMDG-Cl conditions (pH 7.4), and pipette solution contained 5 mM Ca2+ and bath solution was calcium free. (A) Representative current recordings at the Vm values are given on the right side of each trace. The dashed lines indicate the closed channel current levels (C-). Channel unitary conductance and reversal potential in these conditions (pHi 7.4) are given in Table S1. (B) Mean N′Po/Vm relationship. N′Po data were normalized to the respective N′Po at Vm 80 mV. Each point is the mean of 6–10 measurements, and SEM is shown as error bars. The continuous line is a nonlinear least squares fit with the Boltzmann equation of mean normalized data.