Figure 5.

Treatment with the anti-human NKG2A monoclonal antibody transiently depleted HLA-E⁺ autologous myeloid, B, T, NK and DC subpopulations in NSG mice engrafted with human CD34⁺ hematopoietic stem cells. After 3.5 Gy total body irradiation, mice were infused with human CD34⁺ hematopoietic stem cells. One month after, when NKG2A⁺ NK cells differentiated from CD34⁺ cells reached a plateau value, mice were treated with 300 μg of anti-human NKG2A monoclonal antibody. Transient depletion of human myeloid, B, dendritic, and NK cell subpopulations in the bone marrow* (A) and spleen (B) and double negative (DN), single CD8⁺ or CD4⁺, CD4⁺/CD8⁺ double positive (DP) thymocytes (C) was followed by recovery of all cell subsets within 10 days. We pooled results of three experiments with five mice per group for each experiment. *Bone marrow cell numbers are from two femurs per mouse.