Figure 1.

Sustained engraftment of xenotransplanted juvenile myelomonocytic leukemia (JMML) cells in Rag2^−/−γc^−/− mice. Spleen mononuclear cells (MNC) from JMML Patients #1 to #5 were transplanted into sublethally irradiated mice (1×10⁶ cells per mouse via intrahepatic injection or 5×10⁶ cells per mouse via intravenous injection). Hematopoietic cells were obtained from indicated organs at 7–37 weeks post transplant. Human cell engraftment as assessed by flow cytometry of CD45⁺ cells is shown for animals transplanted from Patient #1 (n=31 mice), Patient #2 (n=37 mice), Patient #3 (n=10 mice) and Patient #5 (n=4 mice). The level of human engraftment was defined as proportion of human CD45⁺ cells within the total population of murine and human CD45⁺ cells. Cells from Patient #4 consistently failed to engraft (n=9 mice). The dotted line represents the definition of successful engraftment (≥0.5% human CD45⁺ cells).