Figure 2.

Organ infiltration and human cell subpopulations after xenotransplantation of juvenile myelomonocytic leukemia (JMML) cells in Rag2^−/−γc^−/− mice. (A) Hematopoietic cells were obtained from indicated organs at 7–37 weeks after transplantation from 4 patients with JMML (n=58 mice). Cell subpopulations were assessed by flow cytometry with antibodies to human CD45, CD34, CD33, CD13, CD15, CD11B, CD19 and CD3. Bars indicate mean value and standard error. (B) Representative example of splenomegaly in mice with JMML cell engraftment (top) and normal spleen size in non-engrafted mice (bottom). The spleen weight of 49 engrafted and 17 non-engrafted mice was measured (right panel).