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Prominent ALDHint population of CD34+CD38− cells from a patient with FLT3-ITD AML. (A) Representative flow cytometric staining pattern of ALDH activity by side scatter (SSC) is displayed for CD34+CD38− cells isolated from patient. (B) FLT3-ITD status of isolated cell fractions. The CD34+CD38−ALDHint population (oval) harbored the FLT3-ITD mutation, while the CD34+CD38−ALDHhigh cells (square) exclusively displayed the 330bp wild-type gene.
