Figure 4.
Subpopulation of phospholipid phosphatidylserine (PS)-exposing platelets in thrombi is transglutaminase positive. Human whole blood was perfused over collagen under anti-coagulated (A) or coagulated (B) conditions at shear rate of 1000 s−1. Blood samples were pre-incubated with vehicle (control) or K9-DON (100 μM). (A and B) Representative microscopic images of differential interference contrast (DIC), and confocal images of AF647-annexin A5 or Rhod-A14 fluorescence (bars, 20 μm). Right bar graphs indicate co-localization coefficient of AF647-annexin A5 with Rhod-A14 fluorescence and of Rhod-A14 with AF647-annexin A5 under vehicle-treated (non)coagulant conditions, as assessed with Zen software. Mean ± SEM (n=4).