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. 2015 Aug;100(8):1005–1013. doi: 10.3324/haematol.2014.121590

Figure 2.

Figure 2.

TIMP-1 does not prolong the survival of circulating neutrophils. (A) Viability of blood neutrophils isolated from AdT1-transduced mice. Neutrophils were isolated from DBA/2 mice (n=5 per group) three days after adenoviral transduction. Viability was analyzed ex vivo in an AlamarBlue assay. (B) Viability of isolated blood neutrophils exposed to recombinant TIMP-1 (rT1). Neutrophils were isolated from untreated DBA/2 mice and incubated with rT1 in AlamarBlue assays. (C and D) Annexin V staining of blood neutrophils in AdT1-transduced mice. Blood samples were taken three days after adenovirus injection, stained immediately with Annexin V and analyzed by flow cytometry. Relative (C) and absolute (C) quantification of apoptotic neutrophils. Columns: mean; Bars: SEM; Student’s t-test. ns: not significant; n=5.