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. 2015 Aug;100(8):1005–1013. doi: 10.3324/haematol.2014.121590

Figure 3.

Figure 3.

TIMP-1 does not directly mobilize neutrophils from the bone marrow. (A and B) Migration of isolated bone marrow (BM) neutrophils ex vivo towards rTIMP-1 (A) or towards FCS after pre-incubation with PBS or rTIMP-1 (B). (C) Mobilization of neutrophils by rTIMP-1 in vivo. rTIMP-1 protein was i.p. injected into DBA/2 mice and blood neutrophils were quantified 2 h later by flow cytometry using trucountTM tubes. Columns: mean; Bars: SEM; Student t-test. ns: not significant; n=5. (D) Quantification of neutrophils in BM. DBA/2 mice were transduced with AdCtrl (white) or AdTIMP-1 (black) and sacrificed 1, 3 or 6 days later. Ly6G-positive cells were immunohistochemically stained on femur sections and quantified per microscopic field. Columns, mean; Bars, SEM; Student t-test: ns: not significant; n=5. (E) BM cells were isolated from DBA/2 mice 3 days after adenoviral transduction. Gating (left) and relative quantification (right) of mature neutrophils (CD11b+ Ly6Ghigh) by flow cytometry. Columns: mean; Bars: SEM; Student’s t-test. ns: not significant; n=5.