Figure 5.

Increased neutrophil production accounts for TIMP-1-induced neutophilia. (A–C) DBA/2 mice (n=5) were transduced with AdTIMP-1 and BrdU was repeatedly injected, starting with elevation of TIMP-1 levels. Three days later, BrdU positive neutrophils in blood were analyzed by flow cytometry. (A) Representative plots (left) and relative quantification (right) of BrdU- and BrdU⁺ blood neutrophils. (B and C) Absolute quantification of BrdU- (B) and BrdU⁺ (C) neutrophils in peripheral blood. (D) Relative quantification of BrdU⁺ neutrophils in BM. Columns: mean; Bars: SEM; Student’s t-test. ns: not significant; *P<0.05; **P<0.01.