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. 2016 Aug 29;214(5):619–635. doi: 10.1083/jcb.201602046

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© 2016 Mauthe et al.

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Figure 9. — Depletion of ATG13 and FIP200 results in higher picornavirus production in host cells. (A) Cells were transfected with siRNA against ATG13, FIP200, ATG7, or scramble siRNA (siCtr) for 48 h, and the number of surviving cells was determined after 24 h of infection with EMCV-Zn (in U2OS cells), EV71 (in U2OS cells), or CVA21 (in HeLa cells) at MOI 0.1 (n = 3). (B) The culture supernatants of the experiment in A were collected, and the virus titer was measured using the TCID₅₀ assay (n = 3). Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; no symbol indicates not significant. siCtr, scramble siRNA.