Figure 1.

kon expression in the CNS. (a and c) kon mRNA distributed in lateral cells in the embryo, including the longitudinal glioblast (arrows), which divides into three to four progeny cells at stage 12 as it migrates into the CNS. (b) Larval abdominal VNC glia. Repo is in all glia except midline glia; the NG are GS2⁺ and comprise the alrmGAL4⁺ Pros⁺ and the NP6520GAL4 Pros⁻ glia; NG in one hemisegment are represented on the left. ElavGAL4 drives expression in all neurons. ml, midline. (d) Anti-Kon colocalized with GS2 in glia (arrowheads) enwrapping the neuropil (horizontal view). (e–g) Anti-Kon in VNC, horizontal views on the left, transverse views on the right. (e) Early, Kon is distributed along FasII⁺ axonal fascicles (arrows). (f) Later, Kon colocalizes with alrmGAL4>UASFlyBow1.1 within (arrows) and around (arrows) the neuropil. (g) In pupae, Kon is prominent within the neuropil (arrows) and also colocalizes with GS2 in cell bodies wrapping the neuropil (arrowheads). (h and i) qRT-PCR. (h) Wild-type (wt) profile of kon transcript levels, in whole embryos, dissected CNS from larvae, pupae, and adult heads, all normalized to embryonic levels. n = 3 replicates per time point. Error bars indicate SD. (i) kon RNAi knockdown in all glia caused a significant reduction in kon transcript levels in wandering larva. n = 3 replicates per genotype. One-way ANOVA, P < 0.05; post hoc Sidak test, *, P < 0.05. n.s., not significant. For sample sizes and statistics details, see Table S1; >, GAL4/UAS. Bars: (a) 20 µm; (c and d) 10 µm; (e–g) 50 µm.