Figure 2.

Kon promotes glial proliferation. (a–d) Automatic counting of Repo⁺ or YFP⁺ cells in vivo, throughout the abdominal CNS in 3D using DeadEasy Larval Glia software. (a) Drawing represents the larval VNC: neuronal somata (blue) occupy all nonglial space in cortex; glial nuclei within cortex shown in dark green. kon knockdown with RNAi in all glia (with repoGAL4) reduces Repo⁺ glial number. Kruskal–Wallis: **, P < 0.005. (b) Overexpression of kon in all glia increases Repo⁺ glial number. Mann–Whitney U test: **, P < 0.01. (c) Overexpression of kon in all glia increases YFP reporter glial number. Student’s t test: ***, P < 0.000. (d) Temporal restriction of kon overexpression to the larva only, in neurons, all glia, and only Pros⁺ alrmGAL4⁺ glia, increased Repo⁺ glial cell number. One-way ANOVA, post hoc Dunnett comparisons to control: **, P < 0.01. (e) Overexpression of kon in all glia increased Ebony⁺ NG cell number. Student’s t test: ***, P < 0.001. (f) The number of Ebony⁺ NG that incorporated the S-phase marker BrdU (arrowheads) increased when kon was overexpressed, particularly in Pros⁺ alrmGAL4⁺ NG. Welch’s ANOVA: *, P < 0.05. Sample types are larval VNCs, n = 6–24 per genotype. For details on sample sizes and statistics, see Table S1; > denotes GAL4/UAS. Bars: (a–e) 50 µm; (f) 10 µm.