Figure 6.

Crush injury in living larvae induces the glial regenerative response. (a) Illustration of crushing injury in living, undissected larvae carrying the G9-GFP CNS reporter. Arrowheads in image on the right indicate typical lesion. (b) Crush injury induces a typical progression of wound expansion followed by shrinkage. The lesions are indicated in green. One-way ANOVA, post hoc multiple comparisons Bonferroni test: ***, P < 0.001; **, P < 0.01. (c) Crush injury induced the incorporation of the S-phase marker BrdU into Repo⁺ NG cells (arrowheads). Mann–Whitney U test: **, P < 0.005. Horizontal views on the left, transverse views on the right. (d and e) Crush injury induced glial activation. (d) Neuropil holes were invaded by GS2⁺ glial processes (arrowheads). (e) NG enlarged and acquired thicker projections. (b and c) Sample types are larval VNCs; (b) n = 4–11; (c) n = 8 and 7. For further details, see Table S1; >, GAL4/UAS. Bars: (b and c) 50 µm; (d and e) 10 µm.